Supplementary Online Information for Bizior et al. (2026)

Introduction

This site provides supplementary information to help enable reproduction and independent exploration of the statistical analyses reported in Bizior et al. (2026).

The pages in the Analyses section describe regression modelling for the manuscript “A bacterial Rhesus transporter retunes a structurally conserved ammonium pore into a reversible nitrogen valve” by Bizior et al. We analyse this data to establish evidence in support of answers to the following key questions:

1. Whether the two WT control datasets from solvent-exchange and variant comparison can be pooled.
2. Whether, in the H2O condition, there are effects on maximum amplitude or decay rate constant due to changes in lipid-protein ratio or the D162A amino acid substitution.
3. Whether there are effects on maximum amplitude or decay rate constant due to H170 amino acid substitutions.

Getting Started

The analysis is presented across five pages that can be accessed via the Analyses menu or the links below:

• WT control comparisons
• Figure 2
• Figure 3
• Figure 5

Datasets

The underlying data for these analyses can be downloaded from the link below

• NeRh50_Table_S1_raw_data_used_in_figures.csv

The datasets are presented in Table 1.

# Use helper script to load dataframes
source("assets/r/load_dataframes.R")
dfms <- load_dataframes()

# Show interactive tables of data
DT::datatable(dfms$dfm_wt_amp, options=list(pageLength=5))
DT::datatable(dfms$dfm_wt_k, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig2_amp, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig2_k, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig3_amp, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig3_k, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig5_amp, options=list(pageLength=5))
DT::datatable(dfms$dfm_fig5_k, options=list(pageLength=5))

Table 1: Raw data (maximum amplitude, decay rate) for the manuscript analyses

(a) WT maximum amplitude data under solvent exchange conditions

(b) WT rate data under solvent exchange conditions

(c) Figure 2 maximum amplitude data for comparison with D162A and H170 variants

(d) Figure 2 rate data for comparison with D162A and H170 variants

(e) Figure 3 D162A variant maximum amplitude data

(f) Figure 3 D162A variant rate data

(g) Figure 5 H170 variant maximum amplitude data

(h) Figure 5 H170 variant rate data

Reporting Problems Or Making Suggestions

Please report any issues, problems, or suggested enhancements at the issues page